Non-enzymatic Amperometric Glucose Biosensor Based on Copper Hexacyanoferrate-film Modified-gnp-graphite Composite Electrode

A non-enzymatic amperometric glucose biosensor based on a modified electrode was fabricated by electrochemical derivatization of copper hexacyanoferrate (CuHCF) film at gold nanoparticles (GNP) adsorbed graphite wax composite electrode. The modified electrode was characterized by cyclic voltammetry and electrochemical impedance spectroscopy (EIS). The electrode was also characterized by scanning electron microscopy (SEM) which indicated that the CuHCF particles sized at 100-120 nm were dispersed uniformly on electrode surface. The modified electrode is exemplified towards the non-enzymatic sensing of glucose using cyclic voltammetry and amperometry. A linear calibration curve for glucose determination is obtained in the concentration range from 8.6x10 to 1.2x10M with a detection limit of 1.3x10M. The interference of ascorbic acid has been eliminated by coating the electrode surface with nafion.